
Cataloguenumber
ANT-711
Synonyms
MinichromosomeMaintenanceComplexComponent7,MCM7MinichromosomeMaintenanceDeficient7(S.Cerevisiae),MinichromosomeMaintenanceDeficient(S.Cerevisiae)7,DNAReplicationLicensingFactorMCM7,HomologofS.CerevisiaeCdc47,CDC47Homolog,P1CDC47,PNAS146,P85MCM,MCM2,CDC47,P1.1-MCM3,EC3.6.4.12.
Type
Introduction
MCM7isahighlyconservedmini-chromosomemaintenanceprotein(MCM)vitalforeukaryoticgenomereplicationinitiation.TheMCMproteinsformahexamericproteincomplexwhichisakeycomponentofthepre-replicationcomplex(pre_RC)whichtakespartinreplicationforksformationandinDNAreplicationrelatedproteinsrecruitment.TheMCMcomplexiscomprisedofMCM2,4,6and7proteinsandpossessesDNAhelicaseactivity,suchasDNAunwinding.
Clone
PAT118H7AT.
PhysicalAppearance
Immunogen
Anti-humanMCM7mAb,isderivedfromhybridizationofmouseF0myelomacellswithspleencellsfromBALB/cmiceimmunizedwitharecombinanthumanMCM7protein1-414aminoacidspurifiedfromE.coli.
IgSubclass
MouseIgG1heavychainandklightchain.
PurificationMethod
MCM7antibodywaspurifiedfrommouseasciticfluidsbyprotein-Aaffinitychromatography.
Formulation
1mg/mlcontainingPBS,pH-7.4,10%Glyceroland0.02%SodiumAzide.
StorageProcedures
StABIlity/ShelfLife
Applications
MCM7antibodyhasbeentestedbyELISAandWesternblotanalysistoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.