
Cataloguenumber
ANT-705
Synonyms
GLNS,EC6.3.1.2,EC4.1.1.15,GLUL,GlutamineSynthetase,GS,Glutamatedecarboxylase,Glutamate--ammonialigase,PIG43,PIG59.
Type
Introduction
GLULcatalyzesthesynthesisofglutaminefromglutamateandammonia.Glutamineisamajorsourceofenergyandthattakespartincellproliferation,inhibitionofapoptosis,andcellsignaling.GLULisexpressedduringearlyfetalstages,andhasaroleinmaintainingbodypHbyremovingammoniafromcirculation.MutationsinGLULgenearerelatedwithcongenitalglutaminedeficiency.
Clone
PAT8D7AT.
PhysicalAppearance
Immunogen
Anti-humanGLULmAb,isderivedfromhybridizationofmouseF0myelomacellswithspleencellsfromBALB/cmiceimmunizedwithrecombinanthumanGLULaminoacids1-373purifiedfromE.coli.
IgSubclass
MouseIgG1 heavychainandκlightchain.
PurificationMethod
GLULantibodywaspurifiedfrommouseasciticfluidsbyprotein-Aaffinitychromatography.
Formulation
1mg/mlcontainingPBS,pH-7.4,10%Glyceroland0.02%SodiumAzide.
StorageProcedures
StABIlity/ShelfLife
Applications
GLULantibodyhasbeentestedbyELISAandWesternblotanalysistoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.