
Cataloguenumber
ANT-710
Synonyms
V-AktMurineThymomaViralOncogeneHomolog1,ProteinKinaseBAlpha,Proto-OncogeneC-Akt,RAC-PK-Alpha,EC2.7.11.1,PKBAlpha,CWS6,PKB,RAC,RAC-AlphaSerine/Threonine-ProteinKinase,RacProteinKinaseAlpha,ProteinKinaseB,PKB-ALPHA,RAC-ALPHA,EC2.7.11,AKT1m,PRKBA,AKT,RAC-alphaserine/threonine-proteinkinase.
Type
Introduction
Akt1,alsoknownasAktorelseproteinkinaseB(PKB)isanimportantmoleculeinmammaliancellularsignaling.Inhumans,therearethreegenesinthe"Aktfamily":Akt1,Akt2,andAkt3.Moreover,theseenzymesaremembersoftheserine/threonine-specificproteinkinasefamily(EC2.7.11.1).Akt1isinvolvedincellularsurvivalpathways,byinhibitingapoptoticprocesses.Akt1isalsoabletoinduceproteinsynthesispathways,andisthereforeakeysignalingproteininthecellularpathwayswhichleadtoskeletalmusclehypertrophy,andgeneraltissuegrowth.Sinceitcanblockapoptosis,andtherebypromotecellsurvival,Akt1hasbeenimplicatedasamostimportantfactorinnumeroustypesofcancer.Akt(nowalsocalledAkt1)wasatfirstidentifiedastheoncogeneinthetransformingretrovirus,AKT8.
Clone
PAT6D5AT.
PhysicalAppearance
Immunogen
Anti-humanAKT1mAb,isderivedfromhybridizationofmouseF0myelomacellswithspleencellsfromBALB/cmiceimmunizedwithrecombinanthumanAKT1aminoacids1-480purifiedfromE.coli.
IgSubclass
MouseIgG2aheavychainandκlightchain.
PurificationMethod
AKT1antibodywaspurifiedfrommouseasceticfluidsbyprotein-Aaffinitychromatography.
Formulation
1mg/mlcontainingPBS,pH-7.4,10%Glyceroland0.02%SodiumAzide.
StorageProcedures
StABIlity/ShelfLife
Applications
AKT1antibodyhasbeentestedbyELISAandWesternblotanalysistoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.