
Cataloguenumber
ANT-688
Synonyms
SerpinFamilyEMember1,EndothelialPlasminogenActivatorInhibitor,SerpinE1,PLANH1,PAI-1,PAI1,PAI,PlasminogenActivatorInhibitorTypeI,PlasminogenActivatorInhibitor1,SERPINE1.
Type
Introduction
Plasminogenactivatorinhibitor-1istheprincipalinhibitoroftissueplasminogenactivator(tPA)andurokinase(uPA),theactivatorsofplasminogenandhencefibrinolysis(thephysiologicalbreakdownofbloodclots).Itisaserineproteaseinhibitor(serpin)protein(SERPINE1).TheotherPAI,plasminogenactivatorinhibitor-2(PAI-2)issecretedbytheplacentaandonlypresentinsignificantamountsduringpregnancy.Inaddition,proteasenexinactsasaninhibitoroftPAandurokinase.PAI-1,however,isthemaininhibitoroftheplasminogenactivators.
Clone
PAT1C5AT.
PhysicalAppearance
Immunogen
Anti-humanSERPINE1mAb,isderivedfromhybridizationofmouseF0myelomacellswithspleencellsfromBALB/cmiceimmunizedwithrecombinanthumanSERPINE1aminoacids24-402purifiedfromE.coli.
IgSubclass
MouseIgG1 heavychainandklightchain.
PurificationMethod
SERPINE1antibodywaspurifiedfrommouseasciticfluidsbyprotein-Aaffinitychromatography.
Formulation
1mg/mlcontainingPBS,pH-7.4,10%Glyceroland0.02%SodiumAzide.
StorageProcedures
StABIlity/ShelfLife
Applications
SERPINE1antibodyhasbeentestedbyELISA,WesternblotanalysisandICC/IFtoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.