
Cataloguenumber
ANT-680
Synonyms
Gliamaturationfactorbeta,GMFB,GMF-B,GMF-beta,GMF.
Type
Introduction
GliaMaturationFactor-Betastimulatesdifferentiationofnormalneuronsaswellasglialcells.GMFBinhibitstheproliferationoftheN-18neuroblastomalineandtheC6gliomalinewhilepromotingtheirphenotypicexpression.
GMF-betainhancesthephenotypicexpressionofglia&neuronsthusinhibitstheproliferationoftheirrespectivetumorswhenaddedtocellculture.AlthoughastrocytesproduceGMF-bandstoresitinsidethecells,theydon’tsecretetheGMF-Bintotheculturedmedium.Cell-surfaceGMFbactsonthetargetcellsatcloserangewhencellsareindirectcontact.GMF-BetaisproducedbythymicepithelialcellsandplaysanimportantroleinTcelldevelopmentinfavorofCD4+Tcells.
GMF-Betaisabrain-specificproteinwhichbelongstotheactin-bindingproteins(ADF)family.GMF-betaappearstoplayaroleinthedifferentiation,maintenance,andregenerationofthenervoussystem.Italsosupportstheprogressionofcertainauto-immunediseases,possIBLythroughitsABIlitytoinducetheproductionandsecretionofvariouspro-inflammatorycytokines.
Clone
PAT44D8AT.
PhysicalAppearance
Immunogen
Anti-humanGMFBmAb,isderivedfromhybridizationofmouseF0myelomacellswithspleencellsfromBALB/cmiceimmunizedwithrecombinanthumanGMFBaminoacids1-142purifiedfromE.coli.
IgSubclass
MouseIgG2aheavychainandKappalightchain.
PurificationMethod
GMFBantibodywaspurifiedfrommouseasciticfluidsbyprotein-Aaffinitychromatography.
Formulation
1mg/mlcontainingPBS,pH-7.4,10%Glyceroland0.02%SodiumAzide.
Formulation
1mg/mlcontainingPBS,pH-7.4,10%Glyceroland0.02%SodiumAzide.
StorageProcedures
Stability/ShelfLife
Applications
GMFBantibodyhasbeentestedbyELISA,WesternblotandICC/IFtoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.