
Cataloguenumber
ANT-690
Synonyms
OBProtein,ObesityProtein,OBS,Obesityfactor.
Type
Introduction
A16-kDapeptidehormonesecretedfromwhiteADIpocytesandimplicatedintheregulationoffoodintakeandenergybalance.Leptinprovidesthekeyafferentsignalfromfatcellsinthefeedbacksystemthatcontrolsbodyfatstores.
Clone
PAT7E10AT.
PhysicalAppearance
Immunogen
Anti-humanLeptinmAb,isderivedfromhybridizationofmouseF0myelomacellswithspleencellsfromBALB/cmiceimmunizedwithrecombinanthumanLeptinaminoacids22-167purifiedfromE.coli.
IgSubclass
MouseIgG2b heavychainandklightchain.
PurificationMethod
Leptinantibodywaspurifiedfrommouseasciticfluidsbyprotein-Aaffinitychromatography.
Formulation
1mg/mlcontainingPBS,pH-7.4,10%Glyceroland0.02%SodiumAzide.
StorageProcedures
StABIlity/ShelfLife
Applications
LeptinantibodyhasbeentestedbyELISA,WesternblotanalysisandICC/IFtoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.