Cataloguenumber
PRO-361
Synonyms
Vascularcelladhesionprotein1,V-CAM1,INCAM-100,CD106,VCAM1,L1CAM,MGC99561,DKFZp779G2333.
Introduction
VCAM1isamemberoftheIgsuperfamily.Itisacellsurfacesialoglycoproteinexpressedbycytokineactivatedendothelium.VCAM-1contains6or7immunoglobulindomains,andisexpressedonbothlargeandsmallvesselsonlyaftertheendothelialcellsarestimulatedbycytokines.Theproteinhasanumberoffunctionsincludingtheregulationofleukocytemigration,leukocyteendothelialcelladhesionandsignaltransductionandmayplayaroleinanumberofinflammatorydiseases(artherosclerosisandrheumatoidarthritis).
Description
VCAM1RabbitRecombinantisexpressedininsectcellsandcontaining674aminoacids(a.a.25-698)fusedtoaN-terminalhexahistidinetag,havingatotalMWof77.06kDa.
Source
Baculovirussytem,insectcells.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheVCAM1solution(1mg/ml)contains50mMTris,300mMNaCl,10%Glycerol,pH7.5.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
AFKIETFPESRSLAQIGDSVSLTCTTTGCASPTFSWRTQIDSPLNGKVRSEGTTSTLTMDPVSFENE
HSYLCTATCESKKLEKGIQVEIYSFPKDPEIHLSGPLEVGEPITVKCLVPDVYPFDRLEVDLLKGDYLM
KKQDFLEDMDRKSLETKSLEVTFIPVIEDIGKLIVCRAKLHIDEIDSEPKERETTKELQVYISPKNTVIS
VNPSTRLQEGGSVTMTCSSEGLPVPEIFWSKKQDNGNLQRLSGNATLTLIAMRMEDSGIYVCEG
VNQIGKSRKEVELIVQEKPFTVEISPGPRIAAQIGDPVVLTCSVRGCETPSFSWRTQIDSPLNGQVT
SEGTKSLLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIELSGPPVNGRPVTVSCKVP
NVYPFDXLEIELLKGETMMKNKEFLEEEDKKSLETKSLEMTFIPTMEDTGKVLVCQAKLHIDEMEF
EPKQRQSTQPLFVNVAPRDIAVWVSPSSIVEEGRSVNMTCSSYGLPAPKILWSRQLKNGDLQPLS
ENTTLALISTKLEDSGIYVCEGINLAGKSRKEVELVIQVAPKDIQLTAFPSKSVKEGDTVIISCTCGNV
PETWIILKKKAETGDTVLKSIDGAYTIRKAQLEDAGVYECESKNEVGSQLRSITLDVKGRENSKDYF
SPE
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.