Cataloguenumber
PRO-2458
Synonyms
SialicAcidBindingIgLikeLectin7,QA79MembraneProtein,SIGLEC-7,D-Siglec,AIRM-1, CDw328,AIRM1,P75,SialicAcidBindingIg-LikeLectin19,Pseudogene,AdhesionInhibitoryReceptorMolecule1,Siglec-7,SialicAcidBindingIg-LikeLectin,Pseudogene2,SialicAcidBindingImmunoglobulin-LikeLectin7,AdhesionInhibitoryReceptorMolecule1,SialicAcidBindingIg-LikeLectin7,SialicAcid-BindingIg-LikeLectin7,CD328Antigen,SIGLEC19P,P75/AIRM1,SIGLECP2,CD328,QA79.
Introduction
Sialicacid-bindingIg-likelectin7isoform1,alsoknownasSIGLEC7,isanewlydiscoveredfamilyofsialicacid-bindinglectinsoftheimmunoglobulin(Ig)superfamily.TheextracellularportionhastwoIg-likedomains,withtheamino-terminalV-setIgdomaincontainingaminoacidresidueswhichknowntobeimplicatedinsialicacidrecognitionbyotherSiglecs.ThecombinationofanextracellularsialicacidbindingsiteandanintracellularITIMmotifproposesthatSIGLEC7isinvolvedintrans-membraneregulatorysignalingreactions.FurThermore,onthecellsurfaceSIGLEC7existsasamonomerandisexpressedonnaturalkillercells.
Description
SIGLEC7HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining577aminoacids(19-353a.a.)andhavingamolecularmassof64.2kDa(MolecularsizeonSDS-PAGEwillappearatapproximately70-100kDa).SIGLEC7isexpressedwitha239aminoacidhIgG-His-tagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
SIGLEC7proteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPQKSNRKDYSLTMQSSVTVQEGMCVHVRCSFSYPVDSQTDSDPVHGYWFRAGNDISWKAPVATNNPAWAVQEETRDRFHLLGDPQTKNCTLSIRDARMSDAGRYFFRMEKGNIKWNYKYDQLSVNVTALTHRPNILIPGTLESGCFQNLTCSVPWACEQGTPPMISWMGTSVSPLHPS TTRSSVLTLIPQPQHHGTSLTCQVTLPGAGVTTNRTIQLNVSYPPQNLTVTVFQGEGTASTALGNSSSLSVLEGQSLRLVCAVDSNPPARLSWTWRSLTLYPSQPSNPLVLELQVHLGDEGEFTCRAQNSLGSQHVSLNLSLQQEYTGKMRPVSGVLLLEPKSCDKTHTCPPCPAPELLG GPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
