Cataloguenumber
PRO-2432
Synonyms
SialicAcidBindingIgLikeLectin5,Obesity-BindingProtein2,OB-BindingProtein2,CD33Antigen-Like2,SIGLEC-5,CD33L2,OB-BP2,OBBP2,SialicAcid-BindingImmunoglobulin-LikeLectin5,SialicAcidBindingIg-LikeLectin5,SialicAcid-BindingIg-LikeLectin5,CD170Antigen,CD170,Sialicacid-bindingIg-likelectin5,Siglec-5,CD33antigen-like2,Obesity-bindingprotein2,OB-BP2,OB-bindingprotein2,CD170.
Introduction
SialicAcidBindingIgLikeLectin5,alsoknownasSIGLEC5isamemberoftheimmunoglobulinsuperfamilyandSIGLECfamily.SIGLEC5containsof2Ig-likeC2-typedomainsand1Ig-likeV-typedomain.Inaddition,SIGLEC5isexpressedbymonocytic/myeloidlineagecells.SIGLEC5isexpressedathighlevelsinperipheralbloodleukocytes,spleen,andatlowerlevelsinlymphnode,appendix,pancreas,lung,andthymus.
Description
SIGLEC5HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining667aminoacids(17-441a.a.)andhavingamolecularmassof74.2kDa(MolecularsizeonSDS-PAGEwillappearatapproximately70-100kDa).SIGLEC5isexpressedwitha239aminoacidhIgG-HisTagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
SIGLEC5proteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4).
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADLEKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETT RSSELTLTPRPEDHGTNLTCQMKRQGAQVTTERTVQLNVSYAPQTITIFRNGIALEILQNTSYLPVLEGQALRLLCDAPSNPPAHLSWFQGSPALNATPISNTGILELRRVRSAEEGGFTCRAQHPLGFLQIFLNLSVYSLPQLLGPSCSWEAEGLHCRCSFRARPAPSLCWRLEEKPLE GNSSQGSFKVNSSSAGPWANSSLILHGGLSSDLKVSCKAWNIYGSQSGSVLLLQGRSNLGTGVVPAALLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNK ALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
