
Cataloguenumber
PRO-2470
Synonyms
SH2DomainContaining1B,EWS/FLI1-ActivatedTranscript2,EAT-2,EAT2,SH2Domain-ContainingMoleculeEAT2,SH2Domain-ContainingProtein1B,SH2domain-containingprotein1B,EWS/FLI1-activatedtranscript2,EAT-2.
Introduction
SH2domain-containingprotein1B,alsoknownasSH2D1BactivatesNKcells.SH2D1Bparticipatesincontrollingsignaltransductionthroughatleast4receptors,CD84,SLAMF1,LY9andCD244,expressedonthesurfaceofprofessionalantigen-presentingcells.TwoisoformsofthehumanSH2D1Bareproducedbyalternativesplicing.SuperiorMesentericArterySyndromeisoneofthediseaseswhichisassociatedwithSH2D1B.
Description
SH2D1BHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining141aminoacids(1-132a.a.)andhavingamolecularmassof16.4kDa(MolecularsizeonSDS-PAGEwillappearatapproximately18-28kDa).SH2D1Bisexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
SH2D1Bproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and20%glycerol.
StABIlity
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPMDLPYYHGRLTKQDCETLLLKEGVDGNFLLRDSESIPGVLCLCVSFKNIVYTYRIFREKHGYYRIQTAEGSPKQVFPSLKELISKFEKPNQGMVVHLLKPIKRTSPSLRWRGLKLELETFVNSNSDYVDVLPHHHHHH.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.