
Cataloguenumber
PRO-2464
Synonyms
Frizzled-4,Fz-4,hFz4,FzE4,CD344,FZD4.
Introduction
Frizzled-4,alsoknownasFZD4isa7transmembraneglycoproteinwhichbelongstotheFrizzledfamilywithintheG-proteincoupledreceptorsuperfamily.FZD4actsasapositiveregulatoroftheWinglesstypeMMTVintegrationsitesignalingpathway.ThesepathwaysappeartoincludeinteractionswithG-proteins.FZD4isimplicatedintransductionaswellasintercellulartransmissionofpolarityinformationinthecourseoftissuemorphogenesisanddifferentiatedtissues.
Description
FZD4HumanRecombinantproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining428aminoacids(37-222a.a.)andhavingamolecularmassof48.3kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).FZD4isexpressedwitha239aminoacidshIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
FZD4proteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPFGDEEERRCDPIRISMCQNLGYNVTKMPNLVGHELQTDAELQLTTFTPLIQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGMCLSVKRRCEPVLKEFGFAWPESLNCSKFPPQNDHNHMCMEGPGDEEVPLPHKTPIQPGEECHSVGTNSDQYIWVKRSLNCVLKCGYDAGLYSR
SAKEFTDIWLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNG
QPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH