Cataloguenumber
PRO-2481
Synonyms
Sclerostin,SOST,CDD,VBCH.
Introduction
Sclerostin(SOST)isasecretedglycoproteinwithaC-terminalcysteineknot-like(CTCK)domainandsequencesimilaritytotheDAN(differentialscreening-selectedgeneaberrativeinneuroblastoma)familyofbonemorphogeneticprotein(BMP)antagoNISTs.Sclerostinfunctionsasanegativeregulatorofbonegrowth,byinhibitingboneformation.SOSTiswidelyexpressedatlowlevels,withhighestlevelsinthebone,cartilage,kidney,liver,bonemarrowandprimaryosteoblastsdifferentiatedfor21days.SOSTgenedefectscausesclerosteosisandbonedysplasia.
Description
SOSTHumanRecombinantproducedinHEK293cellsisasingle,glycosylatedpolypeptidechain(a.a24-213)containing196aminoacidsincludinga6a.aC-terminalHistag.Thetotalmolecularmassis22.4kDa(calculated).
Source
HEK293Cells.
PhysicalAppearance
Formulation
SOSTfiltered(0.4µm)andlyophilizedfrom0.5mg/mlinPBSand5%(w/v)trehalose,pH7.4.
Solubility
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.SOSTisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StABIlity
Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeafter oneweekat4°C.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
QGWQAFKNDATEIIPELGEYPEPPPELENNKTMNRAENGGRPPHHPFETKDVSEYSCRELHFTRYVTDGPCRSAKPVTELVCSGQCGPARLLPNAIGRGKWWRPSGPDFRCIPDRYRAQRVQLLCPGGEAPRARKVRLVASCKCKRLTRFHNQSELKDFGTEAARPQKGRKPRPRARSAKANQAELENAYHHHHHH.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
