
Cataloguenumber
PRO-2467
Synonyms
RetinoicAcidEarlyTranscript1E,LymphocyteEffectorToxicityActivationLigand,RAE-1-LikeTranscript4,NKG2DL4,N2DL-4,LETAL,ULBP4,RL-4,NKG2DLigand4,BA350J20.7,RAET1E2,N2DL4.
Introduction
RAET1EisamemberoftheMHCclassIfamily.MHCclassIfamilycontainsmainhistocompatibilitycomplex(MHC)classI-relatedgenespositionedinaclusteronchromosome6q24.2-q25.3.RAET1EandRAET1GproteinaredifferentfromotherRAET1proteinssincetheyhavetypeImembrane-spanningsequencesattheirCterminiinsteadglycosylphosphatidylinositolanchorsequences.RAET1EactsasaligandforNKG2Dreceptor,expressedonthesurfaceofnumeroustypesofimmunecells,involvesininnateadaptiveimmunereactions.RAET1EdeliverssignalstoNKcellsandadvancestumorimmunesurveillancebyinducingthegrowthofanti-tumorcytotoxiclymphocyte.
Description
RAET1EHumanRecombinantproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining204aminoacids(31-225a.a.)andhavingamolecularmassof23.4kDa(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).
RAET1Eisexpressedwitha6aminoacidsHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
RAET1Eproteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPHSLCFNFTIKSLSRPGQPWCEAQVFLNKNLFLQYNSDNNMVKPLGLLGKKVYATSTWGELTQTLGEVGRDLRMLLCDIKPQIKTSDPSTLQVEMFCQREAERCTGASWQFATNGEKSLLFDAMNMTWTVINHEASKIKETWKKDRGLEKYFRKLSKGDCDHWLREFLGHWEAMPEPTVSPVNASDIHWSSSSLPDHHHHHH.