
Cataloguenumber
PRO-898
Synonyms
RegulatorofG-proteinsignaling21,RGS21.
Introduction
RegulatorofG-proteinsignaling21(RGS21)belongstotheRGSfamilyandregulatorymoleculeswhichactasGTPaseactivatingproteinsforGalphasubunitsofheterotrimericGproteins.TheRGS21proteininhibitssignaltransductionbyincreasingtheGTPaseactivityofGproteinalphasubunitsthusdrivingthemintotheirinactiveGDP-boundform.
Description
RGS21HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining176aminoacids(1-152a.a.)andhavingamolecularmassof20.2kDa.
RGS21isfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
RGS21proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,30%glyceroland0.1MNaCl.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMPVKCCFYRSPTAETMTWSENMDTLLANQAGLDAFRIFLKSEFSEENVEFWLACEDFKKTKNADKIASKAKMIYSEFIEADAPKEINIDFGTRDLISKNIAEPTLKCFDEAQKLIYCLMAKDSFPRFLKSEIYKKLVNSQQVPNHKKWLPFL.