
Cataloguenumber
PRO-2434
Synonyms
ProgrammedCellDeath1Ligand2,B7DendriticCellMolecule,ProgrammedDeathLigand2,ButyrophilinB7-DC,PDCD1Ligand2,PDCD1L2,B7-DC,CD273,PD-L2,B7DC,PDL2,PD-1-Ligand2,CD273Antigen,PD-1Ligand2,BA574F11.2,Btdc.
Introduction
Programmedcelldeath1ligand2(PDCD1LG2)whichtakespartinthecostimulatorysignal,isnecessaryforT-cellproliferation.PDCD1LG2isalsoinvolvedinIFNGproductioninaPDCD1-independentway.TheinteractionwithPDCD1inhibitsT-cellproliferationbypreventingcytokineproductionandcellcycleprogression.
Description
PDCD1LG2HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining423aminoacids(20-200a.a.)andhavingamolecularmassof47.7kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).
PDCD1LG2isexpressedwitha239aminoacidshIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
PDCD1LG2proteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPLFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.