
Cataloguenumber
PRO-2459
Synonyms
PregnancySpecificBeta-1-Glycoprotein5,Pregnancy-SpecificBeta-1Glycoprotein,FetalLiverNon-SpecificCross-ReactiveAntigen3,Pregnancy-SpecificBeta-1-Glycoprotein5,FL-NCA-3,PS-beta-G-5,PSBG-5,PSG.
Introduction
PSG5belongstothePSGfamily,whichareahighlyconnectedgroupofsecretedglycoproteins,vastlyexpressedinfetalplacentalsyncytiotrophoblastcells.PSGsarecanbefoundinserumfromthefirst2-3weeksofpregnancyandathigherlevelsasthepregnancyprogresses,uptoepointwheretheyarethehighestfetalproteinfoundinmaternalbloodatterm.PSG5’sroleisinducingsecretionofTH2-typecytokinesfrommonocytesandmodulatingthematernalimmunesystemthroughoutthepregnancy,thusdefendingthesemi-allotypicfetusfromrejection.
Description
PSG5HumanRecombinantproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining310aminoacids(35-335a.a.)andhavingamolecularmassof35.1kDa(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).PSG5isexpressedwitha6aminoacidsHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
PSG5proteinsolution(0.25mg/ml)contains20mMTris(pH6.8),40%glyceroland1mMDTT.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADLQVTIEALPPKVSEGKDVLLLVHNLPQNLAGYIWYKGQLMDLYHYITSYVVDGQINIYGPAYTGRETVYSNASLLIQNVTREDAGSYTLHIIKRGDRTRGVTGYFTFNLYLKLPKPYITINNSKPRENKDVLAFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNE
TGPYECEIRDRDGGMRSDPVTLNVLYGPDLPSIYPSFTYYRSGENLYLSCFAESNPPAEYFWTINGKFQQSGQKLSIPQITTKHRGLYTCSVRNSATGKESSKSMTVEVSAPSGIGRLPLLNPIHHHHHH