
Cataloguenumber
PRO-2439
Synonyms
V-setdomain-containingT-cellactivationinhibitor1,B7homolog4,B7-H4,B7h.5,ImmunecostimulatoryproteinB7-H4,ProteinB7S1,T-cellcostimulatorymoleculeB7x,B7H4,VTCN1,B7S1,B7X,PRO1291,RP11-229A19.4.
Introduction
V-SetDomainContainingTCellActivationInhibitor1,alsoknownasVCTN1,ispartoftheB7costimulatoryproteinfamily.Proteinsinthisfamilyarelocatedonthesurfaceofantigen-presentingcellsandinteractwithligandboundtoreceptorsonthesurfaceofTcells.Highleveloftheencodedproteinhasbeenassociatedwithtumorprogression.VCTN1alsotakespartinpromotingepithelialcelltransformation.
Description
VTCN1HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining244aminoacids(25-259a.a.)andhavingamolecularmassof26.9kDa(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).VTCN1isexpressedwitha9aminoacidsHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
VTCN1proteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPLIIGFGISGRHSITVTTVASAGNIGEDGILSCTFEPDIKLSDIVIQWLKEGVLGLVHEFKEGKDELSEQDEMFRGRTAVFADQVIVGNASLRLKNVQLTDAGTYKCYIITSKGKGNANLEYKTGAFSMPEVNVDYNASSETLRCEAPRWFPQPTVVWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTESEIKRRSHLQLLNSKASHHHHHH.