
Cataloguenumber
PRO-191
Synonyms
Vasodilator-stimulatedphosphoprotein,VASP.
Introduction
Vasodilator-stimulatedphosphoprotein(VASP)belongstotheEna-VASPproteinfamily.VASPislinkedwithfilamentousactinformationandlikelyplaysawidespreadroleincelladhesionandmotility.Inaddition,VASPmaybeinvolvedintheintracellularsignalingpathwayswhichregulateintegrin-extracellularmatrixinteractions.
Description
VASPHumanRecombinantfusedwitha20aminoacidHistagatN-terminusproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining363aminoacids(1-343a.a.)andhavingamolecularmassof37.5kDa(MolecularweightonSDS-PAGEwillappearhigher).
TheVASPispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheVASPsolution(0.5mg/ml)contains20mMTris-HClbuffer(pH8.0),5mMDTT,10%glycerol,200mMNaCland0.1mMPMSF.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMSETVICSSRATVMLYDDGNKRWLPAGTGPQAFSRVQIYHNPTANSFRVVGRKMQPDQQVVINCAIVRGVKYNQATPNFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALEGGGPPPPPALPTWSVPNGPSPEEVEQQKRQQPGPSEHIERRVSNAGGPPAPPAGGPPPPPGPPPPPGPPPPPGLPPSGVPAAAHGAGGGPPPAPPLPAAQGPGGGGAGAPGLAAAIAGAKLRKVSKQEEASGGPTAPKAESGRSGGGGLMEEMNAMLARRRKATQVGEKTPKDESANQEEPEARVPAQSESVRRPWEKNSTTLPRMKSSSSVTTSETQPCTPSSSDYSD.