
Cataloguenumber
PRO-2430
Synonyms
Toll-likereceptor2,CD282.
Introduction
TLR2belongstotheToll-likereceptor(TLR)familythatplaysafundamentalroleinpathogenrecognitionandactivationofinnateimmunity.TLRsarehighlyconservedfromDrosophilatohumansandsharestructuralandfunctionalsimilarities.Theyrecognizepathogen-associatedmolecularpatterns(PAMPs)whichareexpressedoninfectiousagents,andmediatetheproductionofcytokinesnecessaryforthedevelopmentofeffectiveimmunity.ThevariousTLRsexhibitdifferentpatternsofexpression.TLR2isexpressedmostabundantlyinperipheralbloodleukocytes,andmediateshostresponsetoGram-positivebacteriaandyeastviastimulationofNF-kappaB.
Description
TLR2MouseRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining805aminoacids(25-587a.a.)andhavingamolecularmassof90.7kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately70-100kDa).
TLR2isexpressedwitha239aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
TLR2proteinsolution(0.25mg/ml)containingPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADLQESLSCDASGVCDGRSRSFTSIPSGLTAAMKSLDLSFNKITYIGHGDLRACANLQVLMLKSSRINTIEGDAFYSLGSLEHLDLSDNHLSSLSSSWFGPLSSLKYLNLMGNPYQTLGVTSLFPNLTNLQTLRIGNVETFSEIRRIDFAGLTSLNELEIKALSLRNYQSQSLKSIRDIHHLTLHLSESAFLLEIFADILSSVRYLELRDTNLARFQFSPLPVDEVSSPMKKLAFRGSVLTDESFNELLKLLRYILELSEVEFDDCTLNGLGDFNPSESDVVSELGKVETVTIRRLHIPQFYLFYDLSTVYSLLEKVKRITVENSKVFLVPCSFSQHLKSLEFLDLSENLMVEEYLKNSACKGAWPSLQTLVLSQNHLRSMQKTGEILLTLKNLTSLDISRNTFHPMPDSCQWPEKMRFLNLSSTGIRVVKTCIPQTLEVLDVSNNNLDSFSLFLPRLQELYISRNKLKTLPDASLFPVLLVMKIRENAVSTFSKDQLGSFPKLETLEAGDNHFVCSCELLSFTMETPALAQILVDWPDSYLCDSPPRLHGHRLQDARPSVLECHQLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.