Cataloguenumber
PRO-276
Synonyms
T-complexprotein1subunitalpha,TCP-1-alpha,CCT-alpha,TCP1,CCT1,CCTA,D6S230E.
Introduction
TCP1isamolecularchaperonethatisamemberofthechaperonincontainingTCP1complex(CCT),alsoknownastheTCP1ringcomplex(TRiC).Thiscomplexconsistsof2identicalstackedrings,eachcontainingeightdifferentproteins.UnfoldedpolypeptidespenetratethecentralcavityofthecomplexandarefoldedinanATP-dependentmanner.TheTCP1proteinisfoundinthecytosolasasubunitofahetero-oligomericchaperone.TCP1hasasignificantfunctioninmaintainingcellularhomoeostasisbyassistingthefoldingofmanyproteinssuchasthecytoskeletalcomponentsactinandtubulin.
Description
TCP1HumanRecombinantfusedwitha20aminoacidHistagatN-terminusproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining576aminoacids(1-556a.a.)andhavingamolecularmassof62.5kDa.TheTCP1ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheTCP1solution(0.5mg/ml)contains20mMTris-HClBuffer(pH8.0),1mMDTT,0.1mMPMSFand10%Glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan80.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSSLGPVGLDKMLVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAELLKNADELVKQKIHPTSVISGYRLACKEAVRYINENLIVNTDELGRDCLINAAKTSMSSKIIGINGDFFANMVVDAVLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESMLISGYALNCVVGSQGMPKRIVNAKIACLDFSLQKTKMKLGVQVVITDPEKLDQIRQRESDITKERIQKILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSLHDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPNTLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTIVKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGALND.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.