
Cataloguenumber
PRO-2417
Synonyms
SRSF1,ASF,SF2,SF2p33,SFRS1,SplicingFactor,Arginine/Serine-Rich,30-KD,A,AlternateSplicingFactor,SRp30a,ASF-1,SerineAndArginineRichSplicingFactor1,Pre-MRNA-SplicingFactorSF2,P33Subunit,SplicingFactor,Arginine/Serine-Rich1,Serine/Arginine-RichSplicingFactor1,Alternative-SplicingFactor1,SRSplicingFactor1,SplicingFactor2.
Introduction
Serine/arginine-richsplicingfactor1(SFRS1)belongstothearginine/serine-richsplicingfactorproteinfamily,andfunctionsinbothconstitutiveandalternativepre-mRNAsplicing.SFRS1bindstopre-mRNAtranscriptsandcomponentsofthespliceosome,andcaneitherinitiateorinhibitsplicingdependingonthepositionofthepre-mRNAbindingsite.TheABIlityofSFRS1toactivatesplicingiscontrolledbyphosphorylationandinteractionswithothersplicingfactorassociatedproteins.
Description
SRSF1HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining254aminoacids(1-248)andhavingamolecularmassof28.5kDa(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).
SRSF1isfusedto6aminoacidHis-TagatC-terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
SRSF1proteinsolution(0.25mg/ml)containing20mMTris-HClbuffer(pH8.0),100mMKCl,1mMDTT,0.2mMEDTA and40%Glycerol.
Stability
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSRSNSRSRSYSPRRSRGSPRYSPRHSRSRSRTHHHHHH.