
Cataloguenumber
PRO-2418
Synonyms
NXPH1,Nbla00697,NPH1,Neurexophilin-1,Neurexophilin1.
Introduction
Neurexophilin1(NXPH1)isapartoftheneurexophilinfamilywhichencodesasecretedproteinwithavariableNterminaldomain,anextremelyconserved,N-glycosylatedcentraldomain,ashortlinkerregion,andacysteine-richCterminaldomain.NXPH1shapesaverytightcomplexwithalphaneurexins,agroupofproteinswhichpromoteadhesionamongdendritesandaxons.
Description
NXPH1HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining259aminoacids(22-271)andhavingamolecularmassof29.7kDa(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).
NXPH1isfusedto9aminoacidHis-TagatC-terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
NXPH1proteinsolution(0.5mg/ml)containingPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPANLTNGGKSELLKSGSSKSTLKHIWTESSKDLSISRLLSQTFRGKENDTDLDLRYDTPEPYSEQDLWDWLRNSTDLQEPRPRAKRRPIVKTGKFKKMFGWGDFHSNIKTVKLNLLITGKIVDHGNGTFSVYFRHNSTGQGNVSVSLVPPTKIVEFDLAQQTVIDAKDSKSFNCRIEYEKVDKATKNTLCNYDPSKTCYQEQTQSHVSWLCSKPFKVICIYISFYSTDYKLVQKVCPDYNYHSDTPYFPSGHHHHHH.