Cataloguenumber
PRO-2461
Synonyms
Nectin-2,HerpesvirusentrymediatorB,HerpesvirusentrymediatorB,HveB,Nectincelladhesionmolecule2,Poliovirusreceptor-relatedprotein2,CD112,HVEB,PRR2,PVRL2.
Introduction
NectinCellAdhesionMolecule2,alsoknownasNECTIN2,isaCa(2+)-independentcell-celladhesionmoleculewhichisoneoftheplasmamembranecomponentsofadherensjunctions.NECTIN2takesavitalpartintheestablishmentofhomotypicaswellasheterotypiccelltocellcontacts.NECTIN2isessentialforresistingherpessimplexvirustype2infectionintransfectedcells.NECTIN2isalsoapossIBLetargetforantibodytherapyofbreast&ovariancancers.NECTIN2mightberelatedwithhumanlongevity.TwodiseaseswhichhavebeenassociatedwithNECTIN2areHerpesSimplexandOvarianCysticTeratoma.
Description
NECTIN2HumanRecombinantproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining337aminoacids(32-360a.a.)andhavingamolecularmassof36.3kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).
NECTIN2isexpressedwithan8aminoacidsHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Formulation
NECTIN2proteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Aminoacidsequence
QDVRVQVLPEVRGQLGGTVELPCHLLPPVPGLYISLVTWQRPDAPANHQNVAAFHPKMGPSFPSPKPGSERLSFVSAKQSTGQDTEAELQDATLALHGLTVEDEGNYTCEFATFPKGSVRGMTWLRVIAKPKNQAEAQKVTFSQDPTTVALCISKEGRPPARISWLSSLDWEAKETQVSGTLAGTVTVTSRFTLVPSGRADGVTVTCKVEHESFEEPALIPVTLSVRYPPEVSISGYDDNWYLGRTDATLSCDVRSNPEPTGYDWSTTSGTFPTSAVAQGSQLVIHAVDSLFNTTFVCTVTNAVGMGRAEQVIFVRETPNTAGAGATGGLEHHHHHH.
