
Cataloguenumber
PRO-204
Synonyms
NucleardistributionproteinnudEhomolog1,NudE,NDE1,NUDE,LIS4,NUDE1,HOM-TES-87.
Introduction
NDE1isacytoplasmicproteinwhichisamemberofthenudEproteinfamily.Phosphorylatedduringmitosis,NDE1iscriticalfortheformationandfunctionofthemitoticspindleinMphaseandfunctionstoregulatetheDynein-mediatedtransportofkinetochoreproteins,aswellascentrosomeduplicationduringinterphase.NDE1isbelievedtointeractwithNDEL1,LIS1andDyneinIC1/2,cytosolicinasignalingpathwaywhichregulatestheformationofneuronsandisfundamentaltothedevelopmentofthecerebralcortex.
Description
NDE1producedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining355aminoacids(1-335a.a.)andhavingamolecularmassof39.9kDa(MolecularweightonSDS-PAGEwillappearhigher).
NDE1isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
StABIlity
NDE1HumanRecombinantalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC.