Prospec/Internalin-A/PRO-031/1mg
商品编号：
PRO-031
品牌：
prospecbio
市场价：
¥54000.00
美元价：
22950.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Catalogue number
PRO-031
Introduction
Internalins are surface proteins located on Listeria monocytogenes. Internalins exist in two known forms, InlA and InlB. Internalins are used by the bacteria to invade mammalian cells via cadherins transmembrane proteins. The precise role of the Internalin proteins and their invasiveness in vivo is not entirely understood. However, in cultured cells, InlA is needed to facilitate Listeria entry into human epithelial cells. While InlB is required for Listeria internalisation in several other cell types, including hepatocytes, fibroblasts, and epithelioid cells.
Description
Internalin Listeria monocytogenes Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 374 amino acids and having a molecular mass of 42.7kDa. The Internalin-A is fused with an 8 amino acid His tag at C-terminus.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Internalin-A is lyophilized from a 0.2µm filtered concentrated solution in 20mM PB pH7.4 and 300mM NaCl.
Solubility
It is recommended to reconstitute the lyophilized Internalin-A in sterile 2XPBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Important note: If the concentration of NaCl is less than 150mM, the protein solution becomes turbid overnight. Please always keep the concentration of NaCl more than 250mM.
Important note: If the concentration of NaCl is less than 150mM, the protein solution becomes turbid overnight. Please always keep the concentration of NaCl more than 250mM.
Stability
Lyophilized Internalin-A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Internalin-A should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE and HPLC.
Amino acid sequence
MANENNFLEI MSETKEIKIV NIPDNNLKKV LNKSLNKSEN SDLTVKDLES IEYLYGIAEN ISNIEGLEYC KNLKILSLQN NDNSKKENFN TITDLSPLKY LKNLVVLDLR NNKISDLSPL ENLTNLESLR LSGNNISNIS PLNKLESLTT LTLSYNEITD ISTISNLKNL THLALYNNKI EDISSLKENT KLQNLSLGFN KIKDISVLSN LKNLYDLSLE ENNIKSIKSL SNLHKLSNIN LKNNKIEDIS PLETCNDFFE KLILDGNRIS DISSLSKKEI IVCSINNQTI TIKDWKVSDN NSFEIKLPND NLDIKIDNIS ENGTFIDNSI KWTNISPKSK LSFEFKQDID NDIHSIFYSG TVRFKRLEHHHH HH.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
品牌介绍
ProSpec-Tany TechnoGene Ltd.公司创立人Dr. Tanchum (Tany) Amarant 主要致力于蛋白生物化学方面的研究工作，1947年出生于以色列。1991-1997创建ReProGen 公司，从事细胞因子和趋化因子的大量生产及纯化；1997-2000创建ProSpec-TechnoGene，主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利United States Patent 5434135。
ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research.
Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more.....
Our research team and scientists have been able to assemble more than 1700 recombinant proteins, peptides and antibodies for your research needs. The proteins are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices.
ProSpec has established sterling reputation among the scientific community, a reputation built on innovative products, customer service and attention to detail. Our reputation for quality is an essential factor in achieving a favorable position in the market place and the benefits associated with manufacturing top quality products.
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