Cataloguenumber
PRO-2476
Synonyms
Endothelialcell-specificmolecule1,ESM-1,ESM1,endocan.
Introduction
Endothelialcell-specificmolecule1(ESM1)isaproteoglycansecretedbyendothelialcells(mostlyinthehumanlungandkidneytissues)anditsmRNAexpressionisregulatedbyinflammatorycytokines.ESM1haspotentimplicationsinlungendothelialcell-leukocyteinteractions.Inaddition,ESM1expressionisdetectedinvariousepitheliaandinADIpocytes.ESM1isinvolvedinangiogenesisanditalsopromotesangiogenicsprouting.ESM1expressionisupregulatedbyTNFalpha,IL1beta,orlipopolysaccharideanddownregulatedbyIFNgamma.GeneticallyengineeredcellsoverexpressingESM1inducetumorformation,implyingthatESM1mightbeinvolvedinthepathophysiologyoftumorgrowthinvivo.
Description
ESM1HumanRecombinantproducedinHEK293cellsisasingle,glycosylatedpolypeptidechain(a.a20-184)containing175aminoacidsincludinga10a.aC-terminalHistag.Thetotalmolecularmassis46.0kDa(calculated).
Source
HEK293cells.
PhysicalAppearance
Formulation
ESM1filtered(0.4µm)andlyophilizedfrom0.5mg/mlinphosphatebufferedsaline.
Solubility
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.ESM1isnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
WSNNYAVDCPQHCDSSECKSSPRCKRTVLDDCGCCRVCAAGRGETCYRTVSGMDGMKCGPGLRCQPSNGEDPFGEEFGICKDCPYGTFGMDCRETCNCQSGICDRGTGKCLKFPFFQYSVTKSSNRFVSLTEHDMASGDGNIVREEVVKENAAGSPVMRKWLNPRHHHHHHHHHH.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.