Cataloguenumber
PRO-2421
Synonyms
DPH2Homolog,S-Adenosyl-L-Methionine:L-Histidine3-Amino-3-Carboxypropyltransferase2,DiphtheriaToxinResistanceProtein2,DiphthamideBiosynthesisProtein2,DPH2L2,DiptheriaToxinResistanceProteinRequiredForDiphthamideBiosynthesis-Like2,2-(3-Amino-3-Carboxypropyl)HistidineSynthaseSubunit2,DiphthamideBiosynthesisProtein2Homolog-Like2,DiphthamideBiosynthesis-LikeProtein2,EC2.5.1.108,DPH2-Like2,DPH2.
Introduction
Diphthamidebiosynthesisprotein2(DPH2)isahomodimerandeachofitsmonomerscanbinda[4Fe-4S]cluster.DPH2isthetargetofADPribosylatingdiphtheriatoxin(DT)andPseudomonasexotoxinA(PE).DPH2wasidentifiedbyitsABIlitytocomplementadiphthamidemutantstrain,andthusservesindiphthamidebiosynthesis.ThelossofDPH2pre-activatesNF-kBanddeathreceptorpathwaysandrendersMCF7cellshypersensitivetotumornecrosisfactor.
Description
DPH2HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining497aminoacids(1-489a.a.)andhavingamolecularmassof53.1kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).DPH2isexpressedwithan8aminoacidsHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
DPH2proteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4),1mMDTTand20%glycerol.
Stability
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MESMFSSPAEAALQRETGVPGLLTPLPDLDGVYELERVAGFVRDLGCERVALQFPDQLLGDAVAVAARLEETTGSKMFILGDTAYGSCCVDVLGAEQAGAQALIHFGPACLSPPARPLPVAFVLRQRSVALELCVKAFEAQNPDPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARDARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPPPAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSGLEHHHHHH.
