Cataloguenumber
PRO-2480
Synonyms
DLK1,Delta-Like1Homolog(Drosophila),PREF1,DLK-1,FA1,DLK,PG2,Delta-LikeHomolog(Drosophila),ProteinDeltaHomolog1,PreADIpocyteFactor1,FetalAntigen1,Secredeltin,DELTA1,Pref-1,ZOG.
Introduction
Delta-Like1,alsoknownasDLK1isamemberoftheNotch/Delta/Serratefamilyofepidermalgrowthfactor(EGF)-likerepeat-containingproteinswhichactasligandsintheNotchsignalingpathway.FurThermore,DLK1hassixhighlyconservedepidermal-growth-factormotifsanditalsocontainsuptotenO-glycosylationandN-glycosylationlocations,sixofthemaredifferentiallyglycosylated.
Description
DLK1HumanRecombinantproducedinHEK293Cellsisasingle,glycosylated,polypeptidechain(a.a24-303)containing290aminoacidsincludinga10a.aC-terminalHistag.Thetotalmolecularmassis31.2kDa(calculated).
Source
HEK293Cells.
PhysicalAppearance
Formulation
DLK1filtered(0.4µm)andlyophilizedfrom0.5mg/mlinPBSand5%w/vtrehalose,pH7.4
Solubility
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.DLK1isnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
AECFPACNPQNGFCEDDNVCRCQPGWQGPLCDQCVTSPGCLHGLCGEPGQCICTDGWDGELCDRDVRACSSAPCANNRTCVSLDDGLYECSCAPGYSGKDCQKKDGPCVINGSPCQHGGTCVDDEGRASHASCLCPPGFSGNFCEIVANSCTPNPCENDGVCTDIGGDFRCRCPAGFIDKTCSRPVTNCASSPCQNGGTCLQHTQVSYECLCKPEFTGLTCVKKRALSPQQVTRLPSGYGLAYRLTPGVHELPVQQPEHRILKVSMKELNKKTPLLTEGQHHHHHHHHHH.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

