
Cataloguenumber
PRO-916
Synonyms
Myosinlightchain5,Myosinregulatorylightchain5,Superfastmyosinregulatorylightchain2,MYLC2,MyLC-2,MYL5.
Introduction
Myosinregulatorylightchain5(MYL5)isahexamericATPasecellularmotorprotein.Myosiniscomprisedof2heavychains,2nonphosphorylatablealkalilightchains,and2phosphorylatableregulatorylightchains.MYL5isaregulatorylightchainandisexpressedinthefetalmuscleandintheadultretina,cerebellum,andbasalganglia.ThereconstitutionofmyosinwithMYL5oralkalilightchainincreasesfilamentvelocitytointermediaterates,andthere-additionofbothclassesoflightchainsfullyreinstatestheoriginalslidingpace.
Description
MYL5HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining156aminoacids(1-132a.a.)andhavingamolecularmassof17.4kDa.
MYL5isfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
MYL5proteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0)containing1mMDTT,30%glyceroland0.1MNaCl.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMDQNRDGFIDKEDLKDTYASLGKTNVKDDELDAMLKEASGPINFTMFLNLFGEKLSGTDAEETILNAFKMLDPDGKGKINKEYIKRLLMSQADKMTAEEVDQMFQFASIDVAGNLDYKALSYVITHGEEKEE.