
Cataloguenumber
PRO-943
Synonyms
Myosinlightchain5,Myosinregulatorylightchain5,Superfastmyosinregulatorylightchain2,MYLC2,MyLC-2,MYL5.
Introduction
Myosinregulatorylightchain5(MYL5)isahexamericATPasecellularmotorprotein.Myosiniscomprisedof2heavychains,2nonphosphorylatablealkalilightchains,and2phosphorylatableregulatorylightchains.MYL5isaregulatorylightchainandisexpressedinthefetalmuscleandintheadultretina,cerebellum,andbasalganglia.ThereconstitutionofmyosinwithMYL5oralkalilightchainincreasesfilamentvelocitytointermediaterates,andthere-additionofbothclassesoflightchainsfullyreinstatestheoriginalslidingpace.
Description
MYL5HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining197aminoacids(1-173a.a.)andhavingamolecularmassof22.1kDa.
MYL5isfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
MYL5proteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0),20%glycerol,0.1MNaCl,1mMDTTand0.1mMPMSF.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMASRKTKKKEGGALRAQRASSNVFSNFEQTQIQEFKEAFTLMDQNRDGFIDKEDLKDTYASLGKTNVKDDELDAMLKEASGPINFTMFLNLFGEKLSGTDAEETILNAFKMLDPDGKGKINKEYIKRLLMSQADKMTAEEVDQMFQFASIDVAGNL
DYKALSYVITHGEEKEE.