Cataloguenumber
PRO-934
Synonyms
MitoticspindleassemblycheckpointproteinMAD2A,HsMAD2,Mitoticarrestdeficient2-likeprotein1,MAD2-likeprotein1,MAD2L1,MAD2.
Introduction
MAD2L1-bindingprotein(MAD2L1)isacomponentofthemitoticspindleassemblycheckpointthatpreventstheonsetofanaphaseuntilallchromosomesareproperlyalignedatthemetaphaseplate.MAD2L1isvitalfortheexecutionofthemitoticcheckpointwhichmonitorstheprocessofkinetochore-spindleattachmentandinhibitstheactivityoftheanaphasepromotingcomplexbysequesteringCDC20untilallchromosomesarealignedatthemetaphaseplate.
Description
MAD2L1HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining225aminoacids(1-205a.a.)andhavingamolecularmassof25.7kDa.
MAD2L1isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
MAD2L1proteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0),20%glycerol,0.1MNaCland1mMDTT.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSMVAYKIPVND.