Cataloguenumber
PRO-2474
Synonyms
KillerCellLectinLikeReceptorK1,KillerCellLectin-LikeReceptorSubfamilyK,Member1,NKG2-D-ActivatingNKReceptor,NKCellReceptorD,D12S2489E,NKG2D,DNASegmentOnChromosome12(Unique)2489ExpressedSequence,KillerCellLectin-LikeReceptorSubfamilyKMember1,NKG2-DTypeIIIntegralMembraneProtein,CD314Antigen,NKG2-D,CD314,KLR.
Introduction
KillerCelllectin-LikeReceptorSubfamilyK,Member1(KLRK1)isanactivatingreceptorwhichhasrecentlygeneratedconsiderableinterest.Themostfascinatingofthese,areacoupleofcloselyrelatedproteinsknownasMICAandMICB.Thesearecell-surfacemoleculesdistantlyrelatedtoMHCclassIproteins,andtheirgeneshaveelementsofheatshockpromoters.Thus,MICAandMICBareexpressedinthecourseofcellstressandareup-regulatedintumorcellsandduringviralinfections.Thisreceptor-ligandcombinationhasacrucialroleintheimmuneresponsetovariouspathologies.
Description
KLRK1HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining386aminoacids(73-216a.a.)andhavingamolecularmassof43.9kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).
KLRK1isexpressedwitha239aminoacidshIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
KLRK1proteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPIWSAVFLNSLFNQEVQIPLTESYCGPCPKNWICYKNNCYQFFDESKNWYESQASCMSQNASLLKVYSKEDQDLLKLVKSYHWMGLVHIPTNGSWQWEDGSILSPNLLTIIEMQKGDCALYASSFKGYIENCSTPNTYICMQRTVVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
