Cataloguenumber
PRO-2448
Synonyms
KillerCellImmunoglobulinLikeReceptor,TwoIgDomainsAndLongCytoplasmicTail5A,KillerCellImmunoglobulin-LikeReceptor,TwoDomains,LongCytoplasmicTail5A,KIR2DL5,CD158F,KillerCellImmunoglobulin-LikeReceptor,TwoDomains,LongCytoplasmicTail,5,KillerCellImmunoglobulin-LikeReceptorKIR2DL5A,KillerCellImmunoglobulin-LikeReceptor2DL5A,CD158f1Antigen,KIR2DL5.1,KIR2DL5.3,CD158F1,KIR2DL5A.
Introduction
Killercellimmunoglobulin-likereceptor2DL5A(KIR2DL5A)isatypeItransmembraneglycoproteinwhichisamemberofthekillercellIg-likereceptor(KIR)family.KIR2DL5Aisdetectedonthecellsurfaceasamonomerwhich,upontyrosinephosphorylation,recruitstheSrchomologyregion2-containingproteintyrosinephosphatase-2.Therefore,KIR2DL5Aisaninhibitoryreceptorgatheringacombinationofgenetic,structural,andfunctionalfeaturesuniqueamongKIR,suggestingthatKIR2DL5Aplaysaspecializedroleininnateimmunity.
Description
KIR2DL5AHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining459aminoacids(22-238a.a.)andhavingamolecularmassof50.5kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).KIR2DL5Aisexpressedwitha239aminoacidshIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
KIR2DL5Aproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPHEGGQDKPLLSAWPSAVVPRGGHVTLLCRSRLGFTIFSLYKEDGVPVPELYNKIFWKSILMGPVTPAHAGTYRCRGSHPRSPIEWSAPSNPLVIVVTGLFGKPSLSAQPGPTVRTGENVTLSCSSRSSFDMYHLSREGRAHEPRLPAVPSVNGTFQADFPLGPATHGGTYTCFGSLHDSPYEWSDPSDPLLVSVTGNSSSSSSSPTEPSSKTGIRRHVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
