Cataloguenumber
PRO-2423
Synonyms
Glypican3,IntestinalProteinOCI-5,GlypicanProteoglycan3,GTR2-2,MXR7,HeparanSulphateProteoglycan,SecretedGlypican-3,Glypican-3,OCI-5,SGBS1,DGSX,SGBS,SDYS,OCI5,SGB,GPC3.
Introduction
Glypican-3(GPC3)belongstotheglypicanfamily,andishighlyexpressedinthelung,liver,andthekidney.Insometissues,GPC3functionssasatumorsuppressorgeneandanoncofetalprotein.TheGlypican-3proteiniscurrentlyconsideredasatumorMarkerandpotentialtargetforimmunotherapy.Glypican-3bindstoandinhibitsthedipeptidylpeptidaseactivityofCD26,anditcanalsoinduceapoptosisincertaincelltypes.DeletionmutationsintheGPC3genearelinkedwithSimpson-GolABI-Behmelsyndrome,akaSimpsondysmorphiasyndrome.
Description
GPC3HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining544aminoacids(25-559a.a.)andhavingamolecularmassof61.8kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).GPC3isexpressedwitha6aminoacidsHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
GPC3proteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
Stability
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPQPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVHHHHHHH.
