Cataloguenumber
PRO-2431
Synonyms
InducIBLeT-CellCostimulatorLigand,B7-RelatedProtein1,B7Homolog2,B7-LikeProteinGl50,B7Homologue2,B7RP-1,ICOSL,B7-H2,B7RP1,B7H2,TransmembraneProteinB7-H2ICOSLigand,InducibleT-CellCo-StimulatorLigand,CD275Antigen,ICOSLigand,KIAA0653,ICOS-L,CD275,LICOS,GL50,ICOSligand,B7homolog2,B7-H2,B7-likeproteinGl50,B7-relatedprotein1,B7RP-1.
Introduction
InducibleT-CellCostimulatorLigandalsoknownasICOSLG,ispartoftheB7familyofco-stimulatorymoleculesrelatedtoB7-1andB7-2.ICOSLGisatransmembraneglycoproteinwithextracellularIgVandIgCdomains,inadditionitbindstoICOSonactivatedTcells.ThedependentsignalingofICOSLGtakespartinaproliferativeresponse.
Description
ICOSLGproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining480aminoacids(19-256a.a.)andhavingamolecularmassof53.7kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).
ICOSLGisexpressedwitha239aminoacidhIgG-His-tagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
ICOSLGproteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPDTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAATLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
