
Cataloguenumber
PRO-2408
Synonyms
GlycophorinC(GerbichBloodGroup),SialoglycoproteinD,GlycoproteinBeta,Glycoconnectin,Glycophorin-D,PAS-2,GPD34,GPC,Glycophorin-C,CD236Antigen,CD236R,CD236,GYPD,GLPC,GE,Glycophorin-C,Glycoconnectin,Glycophorin-D,GPD,Glycoproteinbeta,PAS-2",SialoglycoproteinD.
Introduction
GlycophorinC,alsoknownasGYPC, isanintegralmembraneglycoprotein.GYPCisaminorspecieswhichiscarriedbyhumanerythrocytes,howeverplaysanimportantroleinregulatingthemechanicalstABIlityofredcells.NumeralglycophorinCmutationshavebeendescribed.TheGerbichandYusphenotypesareduetodeletionofexon3&2,respectively.TheWebb&Duchantigens,alsoidentifiedasglycophorinD,resultfromsinglepointmutationsoftheglycophorinCgene.TheglycophorinCproteinhasveryslighthomologywithglycophorinsA&B.
Description
GYPCHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining66aminoacids(1-57a.a.)andhavingamolecularmassof7.2kDa(MolecularsizeonSDS-PAGEwillappearatapproximately18-28kDa).GYPCisexpressedwitha6aminoacidsHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
GYPCproteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
Stability
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPMWSTRSPNSTAWPLSLEPDPGMASASTTMHTTTIAEPDPGMSGWPDGRMETSTPTIMHHHHHH.