Cataloguenumber
PRO-2426
Synonyms
GlycophorinA(MNSBloodGroup),GlycophorinA(MNBloodGroup),SialoglycoproteinAlpha,MNSialoglycoprotein,PAS-2,GPA,Erythroid-Lineage-SpecificMembraneSialoglycoprotein,RecombinantGlycophorinA-BMiltenberger-DR,GlycophorinA(IncludesMNBloodGroup), Mi.VGlycoprotein(24AA),GlycophorinStaTypeC,GlycophorinA,GPA,GlycophorinErik,GlycophorinMiV,GlycophorinSAT,CD235aAntigen,Glycophorin-A,HGpSta(C), HGpMiXI,CD235a,GPErik,HGpMiV,GPSAT,MNS,MN.
Introduction
GlycophorinsA&B(GYPA&GYPB)arethemainsialoglycoproteinsofthehumanerythrocytemembranewhichcarrytheantigenicdeterminantsfortheMNandSsbloodgroups.AlongwiththeMorNandSorsantigenswhichnormallyoccurinallpopulations,approximately40relatedvariantphenotypeswereidentified.ThesevariantscompriseallthevariantsoftheMiltenbergercomplexandsomeisoformsofSta,aswellasDantu,Sat,He,Mg,anddeletionvariantsEna,S-s-U-andMk.GYPAissignificantforthefunctionofSLC4A1andisnecessaryforhighactivityofSLC4A1.GYPAisinvolvedintranslocationofSLC4A1totheplasmamembrane.GYPAisalsoareceptorfor:theinfluenzavirus,Plasmodiumfalciparumerythrocyte-bindingantigen175(EBA-175);bindingofEBA-175isdependentonsialicacidresiduesoftheO-linkedglycansandisalsoareceptorforHepatitisAvirus(HAV).
Description
GYPAHumanRecombinantproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining81aminoacids(20-91a.a.)andhavingamolecularmassof9.1kDa.(MolecularsizeonSDS-PAGEunderreducingconditions18-28kDa).
GYPAisexpressedwitha9aminoacidsHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
GYPAproteinsolution(1mg/ml)containingPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPLSTTEVAMHTSTSSSVTKSYISSQTNDTHKRDTYAATPRAHEVSEISVRTVYPPEEETGERVQLAHHFSEPEHHHHHH
