Cataloguenumber
PRO-2477
Synonyms
Ephrin-A1,EPLG1,TNFAIP4,LERK1,EFL1,ECKLG,EPH-relatedreceptortyrosinekinaseligand1,ImmediateearlyresponseproteinB61,Tumornecrosisfactoralpha-inducedprotein4,TNFalpha-inducedprotein4,ligandofeph-relatedkinase1,tumornecrosisfactor,alpha-inducedprotein4.
Introduction
EFNA1belongstotheephrin(EPH)family.TheEPHsubfamilyisthebiggestgroupofreceptorproteinkinasesandtheytakepartinvitalnervoussystemfunctionanddevelopment.
Description
EFNA1HumanRecombinantproducedinHEK293cellsisasingle,glycosylatedpolypeptidechain(a.a19-182)containing170aminoacidsincludinga6a.aC-terminalHistag.Thetotalmolecularmassis20.2kDa(calculated).
Source
HEK293cells.
PhysicalAppearance
Formulation
EFNA1filtered(0.4µm)andlyophilizedfrom0.5mg/mlinphosphatebufferedsaline,pH7.5containing5%(w/v)trehalose.
Solubility
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.EFNA1isnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StABIlity
Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeafteroneweekat4°C.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
DRHTVFWNSSNPKFRNEDYTIHVQLNDYVDIICPHYEDHSVADAAMEQYILYLVEHEEYQLCQPQSKDQVRWQCNRPSAKHGPEKLSEKFQRFTPFTLGKEFKEGHSYYYISKPIHQHEDRCLRLKVTVSGKITHSPQAHDNPQEKRLAADDPEVRVLHSIGHSHHHHHH.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.