
Cataloguenumber
PRO-2399
Synonyms
Tumornecrosisfactorreceptorsuperfamilymember27,X-linkedectodysplasin-A2receptor,EDA-A2receptor,EctodysplasinA2Receptor,X-LinkedEctodysplasin-A2Receptor,EDA-A2Receptor,TNFRSF27,XEDAR,TumorNecrosisFactorReceptorSuperfamilyMemberXEDAR,TumorNecrosisFactorReceptorSuperfamilyMember27,EctodysplasinA2IsoformReceptor,EDA-A2R,EDAA2R.
Introduction
EDA2R(ectodysplasinA2receptor)mediatestheactivationoftheNF-kappa-BandJNKpathways.ActivationseemstobemediatedthroughbindingtoTRAF3andTRAF6.Inaddition,MutationsinEDAgiverisetoaclinicalsyndromecharacterizedbylossofhair,sweatglands,andteeth.EDA2RspecificallybindstoEDA-A2isoform.ThisproteinisatypeIIItransmembraneproteinoftheTNFR(tumornecrosisfactorreceptor)superfamily,andcontains3cysteine-richrepeatsandasingletransmembranedomainhoweveritlacksanN-terminalsignalpeptide.Alternativelysplicedtranscriptvariantshavebeenfoundforthisgene.AmongthediseasesassociatedwithEDA2Rareectodermaldysplasia1,hypohidrotic,x-linked,andhypohidroticectodermaldysplasia.
Description
EDA2RHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining380aminoacids(1-138a.a.)andhavingamolecularmassof42.5kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).EDA2Risexpressedwitha242aminoacidhIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
EDA2Rproteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4),20%glyceroland1mMDTT.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleaseavoidfreezethawcycles.
Purity
Aminoacidsequence
ADPMDCQENEYWDQWGRCVTCQRCGPGQELSKDCGYGEGGDAYCTACPPRRYKSSWGHHRCQSCITCAVINRVQKVNCTATSNAVCGDCLPRFYRKTRIGGLQDQECIPCTKQTPTSEVQCAFQLSLVEADAPTVPPQEATLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.