
Cataloguenumber
PRO-757
Synonyms
DyneinlightchainTctex-type1,ProteinCW-1,T-complextestis-specificprotein1homolog,DYNLT1,TCTEL1,TCTEX-1,TCTEX1,CW-1.
Introduction
DyneinlightchainTctex-type1(DYNLT1)isamemberofthedyneinlightchainTctex-typefamily.DYNLT1isadyneinlightchaininvolvedincargobinding.DYNLT1actsasoneofseveralnon-catalyticaccessorycomponentsofthecytoplasmicdynein1complexwhicharebelievedtobeinvolvedinlinkingdyneintocargosandtoadapterproteinsthatregulatedyneinfunction.CytoplasmicdyneinisakeymotorproteincomplexresponsIBLeforminus-end,microtubule-basedmotileprocesses.EachdyneincomplexconsistsoftwoheavychainswhichhaveATPaseandmotoractivities,aswellasagroupofaccessorypolypeptides.
Description
DYNLT1HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining133aminoacids(1-113a.a.)andhavingamolecularmassof14.6kDa.
DYNLT1isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
DYNLT1proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,30%glyceroland0.1MNaCl.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKPFKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI.