
Cataloguenumber
PRO-2405
Synonyms
Introduction
Description
DKK1HumanRecombinantproducedinHEKcellsisaglycoproteincontaining235aminoacidresidues.
DKK1Humanwhichhasamolecularweightof25.8kDaispurifiedbyproprietarychromatographictechniques.
Source
HEK.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
TheDKK1proteinwaslyophilizedfromsolutioncontaining1xPBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedDKK1insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedDKK1althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionDKK1 shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedbyanalysisbySDS-PAGEandHPLCanalysis.
BIOLOGicalActivity
Thespecificactivitywasdeterminedbyitsabilitytoinhibittheproliferation
ofHCT116colorectalcarcinomacells.Approximately40%growthinhibitionwas
achievedataDKK-1concentrationof200ng/ml.
Aminoacidsequence
TLNSVLNSNIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEEC
GTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIE
ETITESFGNDHSTLDGYSRRTTLSSKMYHTKGQEGSVCLRSSDCASGLCCARHFWSKICK
PVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRIQKDHHQASNSSRLHTCQRH.