
Cataloguenumber
PRO-2433
Synonyms
Cystatin-C,Cystatin-3, CST3, CST3
Introduction
Cystatinsareasuperfamilyofcysteineproteinaseinhibitorsfoundinbothplantsandanimals.Theycompriseagroupofproteinaseinhibitors,widelydistributedintissuesandbodyfluids,andformtightcomplexeswithcysteineproteasessuchascathepsinB,H,LandS.CystatinC,asecretedmoleculeofthisfamily,isofinterestfrombiochemical,medicineandevolutionarypointsofview.CystatinC,withmolecularweightof13260Da,iscomposedof120aminoacids,lackscarbohydrateandhastwodisulfidebridgeslocatednearthecarboxylterminus.CystatinCisincreasedinpatientswithmalignantdiseases,andisrelatedtotheinsufficiencyofrenalfunctionandappearstobeabetterMarkerthancreatinine.Ontheotherhand,lowlevelsofcystatinCinvolvecausethebreakdownoftheelasticlaminaeand,subsequently,theatherosclerosisandaBDominalaorticaneurysm.
Description
CST3MouseproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining126aminoacids(21-140a.a.)andhavingamolecularmassof14.2kDa(MolecularsizeonSDS-PAGEwillappearatapproximately13.5-18kDa).
CST3isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
CST3proteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
ATPKQGPRMLGAPEEADANEEGVRRALDFAVSEYNKGSNDAYHSRAIQVVRARKQLVAGVNYFLDVEMGRTTCTKSQTNLTDCPFHDQPHLMRKALCSFQIYSVPWKGTHSLTKFSCKNAHHHHHH
BIOLOGicalActivity
TheIC50valueis<1.0nM.TheinhibitoryfunctionofCystatin3onproteaseactivityofpapainwasmeasuredbyafluorometricassayusingZ-FR-AMCatpH7.5at25°C.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.