
Cataloguenumber
HSP-048
Synonyms
Beta-crystallinA4,Beta-A4crystallin,CRYBA4,MCOPCT4.
Introduction
Beta-crystallinA4(CRYBA4)isamemberofthebeta/gamma-crystallinfamilywhicharethedominantstructuralcomponentsofthevertebrateeyelens.Beta-crystallinsconstructaggregatesofvarioussizesandareabletoself-associatetoformdimersortoformheterodimerswithotherbeta-crystallins.TheCRYBA4genebeingabetaacidicgroupmemberispartofageneclusterwithbeta-B1,beta-B2,andbeta-B3.
Description
CRYBA4HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining216aminoacids(1-196a.a.)andhavingamolecularmassof24.5kDa.
CRYBA4isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSH MTLQCTKSAGPWKMVVWDEDGFQGRRHEFTAECPSVLELGFETVRSLKVLSGAWVGFEHAGFQGQQYILERGEYPSWDAWGGNTAYPAERLTSFRPAACANHRDSRLTIFEQENFLGKKGELSDDYPSLQAMGWEGNEVGSFHVHSGAWVCSQFPGYRGFQYVLECDHHSGDYKHFREWGSHAPTFQVQSIRRIQQ.