Cataloguenumber
PRO-225
Synonyms
PAXIP1-associatedprotein1,PTIP-associatedprotein1,PA1,C16orf53,GAS.
Introduction
C16ORF53isacomponentofaSet1-likemultiproteinhistonemethyltransferasecomplex.C16ORF53interactswithPAXIP1/PTIP;thisinteractionisdirectandisnecessaryfortheassociationwiththerestofthePTIPcomplex.TheC16ORF53proteinhasacrucialroleinmaintaininggenomestABIlity,condensationofchromatinandprogressionthroughmitosis.
Description
C16ORF53producedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining274aminoacids(1-254a.a.)andhavingamolecularmassof29.9kDa(MolecularweightonSDS-PAGEwillappearhigher).
C16ORF53isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
C16ORF53proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),10%glycerol,2mMDTTand0.1MNaCl.
Stability
C16ORF53HumanRecombinantalthoughstableat4°Cfor1week,shouldbestoredbelow
-18°C.
Pleasepreventfreezethawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMSLARGHGDTAASTAAPLSEEGEVTSGLQALAVEDTGGPSASAGKAEDEGEGGREETEREGSGGEEAQGEVPSAGGEEPAEEDSEDWCVPCSDEEVELPADGQPWMPPPSEIQRLYELLAAHGTLELQAEILPRRPPTPEAQSEEERSDEEPEAKEEEEEKPHMPTEFDFDDEPVTPKDSLIDRRRTPGSSARSQKREARLDKVLSDMKRHKKLEEQILRTGRDLFSLDSEDPSPASPPLRSSGSSLFPRQRKY.