Cataloguenumber
PRO-2455
Synonyms
Cadherin2,Cadherin2,Type1,N-Cadherin(Neuronal),NeuralCadherin,N-Cadherin,CDw325,NCAD,CDHN,Calcium-DependentAdhesionProtein,Neuronal,CD325Antigen,N-Cadherin1,Cadherin-2,CD325.
Introduction
Cadherin-2isoform1,alsoknownasCDH2isatransmembrane,homophilicglycoproteinwhichbelongstothecalcium-dependentcelladhesionmoleculefamily.CDH2takespartinneuronsandlateronwasdiscoveredtoparticipateincardiacmuscleandincancermetastasisaswell.TheCDH2losspromotestumOrigenesisthroughreleasingmembrane-boundβ-catenin,andsostimulatingWntsignaling.FurThermore,CDH2appearstobeimplicatedintumordevelopment,howeverthisdiscoveryislimitedinadrenocorticaltumors-ACTs.
Description
CDH2HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining574aminoacids(160-724a.a.)andhavingamolecularmassof62.9kDa(MolecularsizeonSDS-PAGEwillappearatapproximately70-100kDa).
CDH2isexpressedwitha6aminoacidsHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
CDH2proteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and20%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPDWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQATDMEGNPTYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIVANLTVTDKDQPHTPAWNAVYRISGGDPTGRFAIQTDPNSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPPQSTATVSVTVIDVNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLSDPANWLKIDPVNGQITTIAVLDRESPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLDINDNAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRLNGDFAQLNLKIKFLEAGIYEVPIIITDSGNPPKSNISILRVKVCQCDSNGDCTDVDRIVGAGLGTGAHHHHHH.
