
Cataloguenumber
PRO-933
Synonyms
ADP-ribosylationfactor3,ARF3.
Introduction
ADP-ribosylationfactor3(ARF3)belongstothehumanARFgenefamily.Thisfamilyencodessmallguaninenucleotide-bindingproteinswhichstimulatetheADP-ribosyltransferaseactivityofcholeratoxinandhavearoleinvesiculartraffickingandasactivatorsofphospholipaseD.ARF3functionsasanallostericactivatorofthecholeratoxincatalyticsubunit,anADP-ribosyltransferase.ARF3isinvolvedinproteintrafficking;maymodulatevesiclebuddinganduncoatingwithintheGolgiapparatus.TheARF3geneiscomprisedof5exonsand4introns.
Description
ARF3HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining201aminoacids(1-181a.a.)andhavingamolecularmassof22.8kDa.
ARF3isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
ARF3proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),20%glyceroland1mMDTT.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGNIFGNLLKSLIGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLANQLKNKK.