Cataloguenumber
DEN-038
Introduction
Description
Source
Purity
Formulation
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Preparationprotocol
ProtocoltopreparedengueIgG/IgMrapidtest:
1.Prepare532to534nmcolloidgold,conjugationwithin24hoursaftercolloidgoldpreparation;
2.AdjustcolloidgoldODat1.3to1.4,conjugationconcentrationandpHare16to18ug/mlgoldand7.2;
3.Shankingconjugationfor6-8hours,thencentrifugetheconjugationat10,000rpmat18oCfor20min;
4.RemovethesupernatnantasmoreaspossIBLe,addthesuppliedgoldsUSPensionbuffer;
5.Addthesuppliedblockingreagenttothefinalconcentrationto0.4%;
6.Blockingfor1hours,thenaddsucrosetothefinalconcentrationto20%;
7.Dippingtheconjugatedgoldtothegoldpad(donotusesodiumphosphateforgoldpadtreatment);
8.Afterdrying,assemblegoldpad,thesuppliedsamplepadandabsorbentpadoverbackingcard;
9.Add10ulserum/plasmaandaddtwodropsrunningbufferforassay.