Cataloguenumber
ENZ-277
Description
Urease(EC3.5.1.5)isanenzymethatcatalyzesthehydrolysisofureaintocarbondioxideandammonia.Thereactionoccursasfollows:(NH2)2CO+H2O=CO2+2NH3.In1926JamesSumnershowedthatureaseisaprotein.Ureaseisfoundinbacteria,yeastandseveralhigherplants.Characteristics:Activesitemetal:nickel(II);Molecularweight:480kDaor545kDaforJackBeanUrease(calculatedmassfromtheaminoacidsequence);OptimumpH:7.4;OptimumTemperature:60degreesCelsius;Enzymaticspecificity:urea;Inhibitors:heavymetals.Themulti-subunitenzymeusuallyhasa3:3(alpha:beta)stoichiometrywitha2-foldsymmetricstructure(notethattheimageabovegivesthestructureoftheasymmetricunit,onethirdofthetrueBIOLOGicalassembly).AnexceptionalureaseisfoundinHelicobacterpylori,whichcombinesfouroftheregularsixsubunitenzymesinanoveralltetrahedralassemblyof24subunits(a12b12).Thissupra-molecularassemblyisthoughttoconferadditionalstABIlityfortheenzymeinthisorganism,whichfunctionstoproduceammoniainordertoneutralisegastricacid.ThepresenceofureaseisusedinthediagnosisofHelicobacterspecies.
Source
PhysicalAppearance
Formulation
Solubility
Stability
Pleasepreventfreeze-thawcycles.
Purity
(a)AnalysisbyRP-HPLC.
(b)AnalysisbySDS-PAGE.