
Cataloguenumber
ENZ-159
Synonyms
ScavengermRNA-decappingenzymeDcpS,DCS-1,Hint-related7meGMP-directedhydrolase,Histidinetriadproteinmember5,HINT-5,DCPS,DCS1,HINT5,HSPC015,HSL1.
Introduction
ScavengermRNA-decappingenzyme(DCPS)isamemberoftheHITfamily.DCPSisrequiredforthecompletedegradationofmRNAs,bothinnormalmRNAturnoverandinnonsense-mediatedmRNAdecay.ItwasshownthatDCPShydrolyzestheresidualm7GpppNcapstructureafterthecomplete3"–5"degradationofthemRNAbytheexosome.FurThermore,DCPSreleasesm7GMPandisincapableofcleavingcapstructuresattachedtoalongRNAchain.
Description
DCPSHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining357aminoacids(1-337a.a.)andhavingamolecularmassof40.8kDa.
DCPSisfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
DCPSproteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0),20%glyceroland1mMDTT.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMADAAPQLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASGDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRNITLPHLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQQLDDLYLIAICHRRGIRSLRDLTPEHLPLLRNILHQGQEAILQRYRMKGDHLRVYLHYLPSYYHLHVHFTALGFEAPGSGVERAHLLAEVIENLECDPRHYQQRTLTFALRADDPLLKLLQEAQQS.