
Cataloguenumber
ENZ-153
Synonyms
Aspartoacylase-2,AcylaseIII,Aminoacylase-3,ACY-3,HepatitisCviruscore-bindingprotein1,HCBP1,ACY3,ASPA2.
Introduction
Aspartoacylase3(ACY3)belongstotheAspartoacylasesubfamily.ACY3hasavitalroleindeacetylatingmercapturicacidsinkidneyproximaltubules.Aspartoacylase3localizestothecytoplasmofS2andS3proximaltubulesandalsototheapicaldomainofS1proximaltubules.Inaddition,ACY3proteinisexpressedatlowlevelsinthestomach,testis,heart,brain,lungandliver,andcanfunctionasanHCV(HepatitisCvirus)corebindingprotein.
Description
ACY3HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining342aminoacids(1-319a.a.)andhavingamolecularmassof37.6kDa.
ACY3isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
ACY3proteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,10%glyceroland0.1MNaCl.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMCSLPVPREPLRRVAVTGGTHGNEMSGVYLARHWLHAPAELQRASFSAVPVLANPAATSGCRRYVDHDLNRTFTSSFLNSRPTPDDPYEVTRARELNQLLGPKASGQAFDFVLDLHNTTANMGTCLIAKSSHEVFAMHLCRHLQLQYPELSCQVFLYQRSGEESYNLDSVAKNGLGLELGPQPQGVLRADIFSRMRTLVATVLDFIELFNQGTAFPAFEMEAYRPVGVVDFPRTEAGHLAGTVHPQLQDRDFQPLQPGAPIFQMFSGEDLLYEGESTVYPVFINEAAYYEKGVAFVQTEKFTFTVPAMPALTPAPSPAS.